On Tuesday, 19 November 2024, first-semester Dentistry students gathered at the Integrated DLC Laboratory on the 4th floor to participate in an essential practicum session focused on protein testing. Before commencing laboratory work, all students were required to wear lab coats, emphasizing the importance of safety and professionalism in the laboratory environment.
This biochemistry practicum aimed to provide students with hands-on experience regarding biochemical reactions occurring in the human body and the laboratory techniques used in research. One of the key themes of this practicum was protein testing, as proteins are essential nutrients crucial for human health.
Proteins consist of long chains of amino acids connected by peptide bonds. They play a vital role in various bodily functions, including tissue repair, enzymatic activity, and immune response. Understanding the structure and function of proteins is fundamental for students pursuing careers in healthcare and research.
During the practicum, students explored various types of protein testing reactions. One method used was precipitation with heavy metals to determine the isoelectric point of proteins. This technique is essential for understanding how proteins behave in different pH environments, which is crucial for various biological processes.
Another reaction involved alkaloid reagents to identify the isoelectric point of proteins. This method helped students understand the importance of protein solubility and stability, which are critical for their functionality in biological systems. The ability to manipulate and comprehend these properties is a key skill for future biochemists and healthcare professionals.
The practicum also included acid precipitation tests to observe the process of protein denaturation. Denaturation can alter protein structure and function, making it an important concept to grasp, especially in the context of diseases and metabolic disorders.
Additionally, the Hopkins-Cole reaction was performed to detect the presence of indole groups in the amino acid tryptophan. This reaction not only reinforced students' knowledge of amino acid structures but also highlighted the significance of tryptophan in various physiological processes, including serotonin production.
Students also conducted sulfur tests to identify sulfur-containing amino acids, such as methionine and cysteine. These amino acids are crucial for protein synthesis and play significant roles in metabolic pathways, making their identification essential for understanding protein function.
The practicum session concluded with a discussion on the implications of protein testing in medical and research fields. By understanding the biochemical reactions related to proteins, students are better equipped to contribute to advancements in healthcare, aligning with Sustainable Development Goals (SDGs) 3 and 4, which focus on education and health access.
The protein testing practicum not only provided essential laboratory skills but also deepened students' understanding of the biochemical processes underlying human health. As future professionals, they are now better prepared to face challenges in the medical field, promoting a healthier society through education and research.
Contributor: Sri Pujiatun | Author: Al Haqi Insan Pratama
