Cytotoxicity Evaluation of A New Resin Matrix System for Dental Fiber-Reinforced Composites
Siti Sunarintyas1, Widowati Siswomihardjo1, Harsini Suwarsohartono1 and Jukka Pekka Matinlinna2
1Department of Dental Biomaterial, Faculty of Dentistry, Gadjah Mada University, Yogyakarta 55281, Indonesia
2The University of Hong Kong, Faculty of Dentistry, 4/F Dental Materials Science, The Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong
Objectives. The purpose of this study was to determine the cytotoxicity of HDMA-based E-glass FRCs. Methods and Materials. The materials used were bis-GMA (Sigma-Aldrich, USA), MMA (ProSciTech, Australia), HDMA (Esstech, USA), CQ (Esstech, USA), CEMA (Esstech, USA), E-glass fibers (Ahlstrom, Finland) and fibroblast cell line (UGM, Indonesia). Fifteen FRCs (2.0mmx2.0mmx25mm) were prepared and divided into 3 groups. The experiment groups were 78.4wt%HDMA+19.6wt%MMA+1.0wt%CQ+ 1.0wt%CEMA (Exp1-group) and 49.0wt%HDMA+49.0wt%MMA+1.0wt%CQ+ 1.0wt%CEMA (Exp2- group). The Control-group was 78.4wt%bis-GMA+19.6wt%MMA+ 1.0wt%CQ+1.0wt%CEMA. The specimens were milled, diluted in a culture medium, contacted to the cells for 24 h then evaluated by MTT method. Cells viability was analyzed by ANOVA and LSD. Result. The average of cells viability in percentage showed for the Exp1-group was 67.73±0.71, Exp2-group was 66.78±0.20 and Control-group was 64.36±1.63. Analysis by ANOVA revealed significant difference of cells viability among the groups (p<0.01). The LSD test showed significant difference between the Exp1-group and Control-group and between the Exp2-group and Control-group (p<0.01); whilst no significant difference between the Exp1-group and Exp2- group (p>0.05). Conclusions. An experimental HDMA-based E-glass FRCs (the Exp1-group and Exp2-group) revealed significant difference on cells viability with bis-GMA-based E-glass FRCs. The HDMA-based E-glass FRCs was less cytotoxic than bis-GMA-based E-glass FRCs.
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